Microsoft Defender AV must be configured to check for definition updates daily.
This policy setting allows specifying the day of the week on which to check for definition updates. The check can also be configured to run every day or to never run at all. This setting can be configured with the following ordinal number values:
(0x0) Every Day (default)
(0x1) Sunday
(0x2) Monday
(0x3) Tuesday
(0x4) Wednesday
(0x5) Thursday
(0x6) Friday
(0x7) Saturday
(0x8) Never
If this setting is enabled, the check for definition updates will occur at the frequency specified. If this setting is disabled or not configured, the check for definition updates will occur at a default frequency.
How this relates to the STIG checklist: STIG vs SCAP benchmark.