Microsoft Defender AV must scan excluded files and directories during quick scans.
In Microsoft Defender Antivirus, when an exclusion for a file or folder is created, it will generally be skipped during both real-time protection and on-demand scans (including quick scans and full scans). However, with newer releases, the option exists to configure quick scans to include files and directories that are otherwise excluded from real-time protection.
How this relates to the STIG checklist: STIG vs SCAP benchmark.